human liposarcoma cell lines sw872  (ATCC)

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: IL-4-Induced M2 macrophage polarization promotes the progression of LPS. RAW264.7 cells were divided into two groups: control group, IL-4 group. (A) Flow cytometry was used to detect the expression level of CD206 on RAW264.7 cells in each group. (B) ELISA was performed to measure the secretion level of IL-10 in RAW264.7 cells from each group. (C) Western Blot was employed to determine the protein expression level of Arg-1 in RAW264.7 cells of each group. A Transwell co-culture system was utilized, which included two groups: control co-culture group (LPS cells co-cultured with untreated RAW264.7 cells). IL-4 co-culture group (LPS cells co-cultured with RAW264.7 cells pre-treated with IL-4). (D) EdU incorporation assay was used to detect the proliferative activity of SW872 cells and 94T778 cells in each group. (E-F) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 cells and 94T778 cells in each group. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Culture Assay, Cell Culture, Activity Assay, Migration

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: mTORC2 regulates TAM via PPAR-γ/CD36 pathway to promote LPS progression. LPS cells (SW872 and 94T778) were co-cultured with RAW264.7 macrophages, and the groups were divided as follows: control co-culture group, IL-4 co-culture group, IL-4+JR-AB2-011 co-culture group, IL-4+JR-AB2-011+LPA co-culture group. (A) EdU assay was used to detect the proliferation of SW872 and 94T778 cells in each co-culture group. (B-C) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 and 94T778 cells in each co-culture group. Data are presented as the mean±SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Culture, Control, Co-Culture Assay, EdU Assay, Migration

Journal: Cancer Medicine

Article Title: Clinical Significance and Therapeutic Potential of Long Non‐Coding RNA H19 in Soft Tissue Sarcoma

doi: 10.1002/cam4.71305

Figure Lengend Snippet: H19 expression in different sarcoma subtypes. (A) H19 RNA‐seq expression data across cell lines from 38 different cancer types derived from the publicly available CCLE database. (B) H19 RNA‐seq expression data from 31 cancer types in tumor tissue derived from TCGA and GTEx data (SARC = sarcoma; TMP = Transcript Per Million). (C) The expression of H19 in 7 different sarcoma cell lines was measured by qRT‐PCR and normalized to the housekeeper genes GAPDH and U6 ( n = 3; mean ± SD). (D) Representative pictures of RNA in situ hybridization of H19 in the liposarcoma cell line SW872 showing a heterogenous expression pattern.

Article Snippet: The sarcoma cell lines SW872 (Liposarcoma) and SW982 (Synovial Sarcoma) were purchased from the American Type Culture Collection (ATCC; Manassas, CA, USA).

Techniques: Expressing, RNA Sequencing, Derivative Assay, Quantitative RT-PCR, RNA In Situ Hybridization

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: ARNT2-driven transcriptional activation of STRA6 reprograms fatty acid metabolism to promote retroperitoneal liposarcoma progression

doi: 10.1007/s00432-025-06352-1

Figure Lengend Snippet: ARNT2 exhibits a high level of expression in liposarcoma and shows a correlation with unfavorable patient prognosis. A – C Differential expression analyses of ARNT2 in TCGA-GTEx, GSE 30929 and GSE 21122 datasets. D Kaplan—Meier analysis assessed the impact of high and low ARNT2 levels on patients’ overall survival in the TCGA database. E The distant-recurrence-free-survival curves of ARNT2 in GSE 30929. F The metastasis-free survival analysis according to ARNT2 expression in GSE 21050. G WB was utilized to detect the expression of ARNT2 in 10 pairs of retroperitoneal liposarcoma tissues and their matched adipose tissues. H IHC analysis of ARNT2 expression in retroperitoneal liposarcoma tissues and their matched adipose tissues. Scale bar: 20 μm. I H Score demonstrates ARNT2 expression in retroperitoneal liposarcoma and their matched adipose tissues. J Expression of ARNT2 in hADSCs and liposarcoma cell lines SW872 and 94T778 was detected by WB

Article Snippet: Human adipose-derived stem cells (hADSCs) and liposarcoma cell line SW872 were purchased from Procell (Hubei, China).

Techniques: Expressing, Quantitative Proteomics

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: ARNT2-driven transcriptional activation of STRA6 reprograms fatty acid metabolism to promote retroperitoneal liposarcoma progression

doi: 10.1007/s00432-025-06352-1

Figure Lengend Snippet: ARNT2 acts as a driver for the growth of liposarcoma cells. A , B The efficiency of ARNT2 knockdown in SW872 and 94T778 cells was confirmed through WB and qPCR assays. C CCK8 assays demonstrated that ARNT2 knockdown led to a suppression of the viability of SW872 and 94T778 cells. D , E Transwell invasion assays and wound healing assays substantiated that ARNT2 knockdown resulted in the attenuation of the invasive and migratory abilities of SW872 and 94T778 cells. Scale bar in D: 50 μm. Scale bar in E: 100 μm. F Colony formation assays indicated that cellular proliferation capacity was reduced following ARNT2 knockdown

Article Snippet: Human adipose-derived stem cells (hADSCs) and liposarcoma cell line SW872 were purchased from Procell (Hubei, China).

Techniques: Knockdown

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: ARNT2-driven transcriptional activation of STRA6 reprograms fatty acid metabolism to promote retroperitoneal liposarcoma progression

doi: 10.1007/s00432-025-06352-1

Figure Lengend Snippet: ARNT2 promotes fatty acid metabolism in liposarcoma cells. A The heatmap was generated by grouping liposarcoma samples in the TCGA database based on high and low expression levels of ARNT2. B , C Gene Set Enrichment Analysis was performed by grouping samples according to the expression levels of ARNT2. D , E WB and qPCR analyses were conducted to assess the alterations in fatty acid metabolism-related genes following ARNT2 knockdown. F , G Oil Red O staining was utilized to evaluate the changes in intracellular lipid droplet content upon ARNT2 knockdown or overexpression. Scale bar: 50 μm. H Triglyceride (TG) content in SW872 and 94T778 cells after ARNT2 knockdown

Article Snippet: Human adipose-derived stem cells (hADSCs) and liposarcoma cell line SW872 were purchased from Procell (Hubei, China).

Techniques: Generated, Expressing, Knockdown, Staining, Over Expression

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: ARNT2-driven transcriptional activation of STRA6 reprograms fatty acid metabolism to promote retroperitoneal liposarcoma progression

doi: 10.1007/s00432-025-06352-1

Figure Lengend Snippet: ARNT2 promotes the growth of liposarcoma in vivo. A Verification of knockdown efficiency of shARNT2 in SW872 cell line by Western blot. B Subcutaneous xenograft tumors derived from the shNC and shARNT2 groups. C , D Weight and growth curves of xenograft tumors. E The expression of ARNT2 and Ki-67 in the xenograft tumors was detected by IHC. Scale bar: 50 μm. F The schematic diagram illustrating the mechanism by which ARNT2 regulates fatty acid metabolism and tumorigenesis in liposarcoma

Article Snippet: Human adipose-derived stem cells (hADSCs) and liposarcoma cell line SW872 were purchased from Procell (Hubei, China).

Techniques: In Vivo, Knockdown, Western Blot, Derivative Assay, Expressing